Mayeso a HIV (I+II) Antibody (mizere iwiri)

Kachilombo ka HIV (I+II) Antibody Test(Mizere iwiri) Chithunzi Chowonetsedwa

Mayeso a HIV (I+II) Antibody (mizere iwiri)

Mtundu: Mapepala Osadulidwa

Chizindikiro: Bio-mapper

Zithunzi za RF0171

Chitsanzo:Mkodzo

Kachilombo ka Edzi, komwe kamatchedwanso human immunodeficiency virus (HIV), ndi kachilombo kamene kamatha kuwononga ma T4 lymphocytes, mbali yofunika kwambiri ya chitetezo cha mthupi cha munthu.Ma antibodies a HIV (HIV AB) amakhala m'magazi a anthu omwe ali ndi kachilombo ka HIV, kaya ali ndi zizindikiro kapena ayi.Chifukwa chake, kuzindikirika kwa HIV AB ndi chizindikiro chofunikira pakuzindikiritsa kachilombo ka HIV.Kuti mudziwe ngati munthu ali ndi kachilombo ka HIV, njira yanthawi zonse yoyezera magazi ndiyo kupita kuzipatala kuti akayezetse magazi a antibody.Muyezo wamba woyezetsa HIV Ab ndi kuyesa kwa antibody mu seramu.Pali njira zambiri zowonera kachilombo ka HIV Ab kunyumba ndi kunja, zomwe zitha kugawidwa m'ma enzyme olumikizidwa ndi immunosorbent assay, agglutination assay ndi immunochromatography molingana ndi mfundo zodziwikiratu.Pogwira ntchito, ma enzyme olumikizana ndi immunosorbent assay, gelatin agglutination test ndi ma reagents osiyanasiyana ozindikira mwachangu amagwiritsidwa ntchito.

Titumizireni imelo

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Kufotokozera mwatsatanetsatane

Western blot (WB), strip immunoassay (LIATEK HIV Ⅲ), radioimmunoprecipitation assay (RIPA) ndi immunofluorescence assay (IFA).Njira yoyesera yovomerezeka yomwe imagwiritsidwa ntchito ku China ndi WB.

(1) Western blot (WB) ndi njira yoyesera yomwe imagwiritsidwa ntchito kwambiri pozindikira matenda ambiri opatsirana.Pankhani ya etiological matenda a kachirombo ka HIV, ndi njira yoyamba yoyesera yotsimikizira yomwe imagwiritsidwa ntchito kutsimikizira ma antibodies.Zotsatira zodziwikiratu za WB nthawi zambiri zimagwiritsidwa ntchito ngati "golide" kuti azindikire zabwino ndi zoyipa za njira zina zoyesera.
Njira yoyesera yotsimikizira:
Pali HIV-1/2 mitundu yosakanikirana ndi mtundu umodzi wa HIV-1 kapena HIV-2.Choyamba, gwiritsani ntchito HIV-1/2 mix reagent kuyesa.Ngati mukunena kuti mulibe, nenani kuti ma antibody omwe ali ndi kachilombo ka HIV alibe;Ngati ali ndi HIV, adzanena kuti ali ndi kachilombo ka HIV-1;Ngati njira zabwino sizikukwaniritsidwa, zimaganiziridwa kuti zotsatira zoyezetsa kachilombo ka HIV sizikudziwika.Ngati pali chizindikiro cha HIV-2, muyenera kugwiritsa ntchito HIV-2 immunoblotting reagent kuchita HIV 2 antibody chitsimikiziro mayeso kachiwiri, zomwe zimasonyeza negative anachita, ndi kunena kuti HIV 2 oteteza chitetezo ndi alibe;Ngati ali ndi HIV, adzanena kuti ali ndi kachilombo ka HIV-2, ndikutumiza chitsanzocho ku labotale yowunikira dziko lonse kuti ifufuze motsatizana ndi nucleic acid.
Kukhudzika kwa WB nthawi zambiri sikutsika poyerekeza ndi kuyesa koyambirira, koma kutsimikizika kwake ndikokwera kwambiri.Izi makamaka zimachokera pa kulekanitsa, ndende ndi kuyeretsedwa kwa zigawo zosiyana za HIV antigen, zomwe zimatha kuzindikira ma antibodies motsutsana ndi zigawo zosiyana za antigen, kotero njira ya WB ingagwiritsidwe ntchito pozindikira kulondola kwa kuyesa koyambirira.Zitha kuwoneka kuchokera ku zotsatira zoyesa zotsimikizira za WB kuti ngakhale kuti ma reagents omwe ali ndi khalidwe labwino amasankhidwa kuti ayesedwe koyambirira, monga m'badwo wachitatu wa ELISA, padzakhalabe zizindikiro zabodza, ndipo zotsatira zolondola zitha kupezeka kokha kupyolera mu mayeso otsimikizira. .
(2) Kuyeza kwa Immunofluorescence (IFA)
Njira ya IFA ndiyopanda ndalama, yosavuta komanso yachangu, ndipo yalimbikitsidwa ndi FDA kuti ipeze zitsanzo za WB zosatsimikizika.Komabe, ma microscopes okwera mtengo a fulorosenti amafunikira, akatswiri ophunzitsidwa bwino amafunikira, ndipo zotsatira zowunikira ndi kutanthauzira zimakhudzidwa mosavuta ndi zinthu zomwe zimakhudzidwa.Zotsatira siziyenera kusungidwa kwa nthawi yayitali, ndipo IFA sayenera kuchitidwa ndikugwiritsidwa ntchito m'ma laboratories wamba.
Lipoti la zotsatira zoyezetsa ma antibody
Zotsatira zakuyezetsa magazi a antibody zidzafotokozedwa mu Table 3.
+Tsatirani njira zodziwira kuti muli ndi HIV 2 antibody, nenani kuti "HIV 2 antibody positive (+)", ndipo chitani ntchito yabwino yowonana pambuyo poyezetsa, chinsinsi komanso miliri ngati pakufunika.
(2) Gwirizanani ndi njira zodziwira kuti mulibe kachilombo ka HIV, ndikunena za “HIV antibody negative (-)”.Ngati mukukayikira kuti muli ndi matenda a "window period", kuyezetsanso kachilombo ka HIV kumalimbikitsidwa kuti muzindikire matendawo mwachangu.
(3) Gwirizanani ndi mfundo za kusatsimikizika kwa ma antibody, nenani "kukayikakayika kwa antibody (±)", ndipo zindikirani m'mawu akuti "dikirani kuyesedwanso pakatha milungu inayi".