CHIDULE NDI KUTANTHAUZA MAYESERO
Leptospirosis imapezeka padziko lonse lapansi ndipo ndi vuto la thanzi labwino kwa anthu ndi nyama, makamaka m'madera omwe ali ndi nyengo yotentha komanso yachinyontho.Malo osungirako zachilengedwe a leptospirosis ndi makoswe komanso mitundu yambiri ya zinyama zoweta.Matenda a anthu amayamba ndi L. interrogans, membala wamtundu wa Leptospira.Matendawa amafalitsidwa kudzera mkodzo kuchokera ku chiweto.
Pambuyo pa matenda, ma leptospires amapezeka m'magazi mpaka atachotsedwa patatha masiku 4 mpaka 7 atapanga anti-L.interrogans ma antibodies, poyamba a gulu la IgM.Chikhalidwe cha magazi, mkodzo ndi cerebrospinal madzimadzi ndi njira yothandiza yotsimikizira kuti matendawa alipo pa 1st mpaka masabata a 2 atakhudzidwa.Kuzindikira kwa serological kwa antiL.interrogans ma antibodies ndi njira yodziwika bwino yodziwira matenda.Mayesero akupezeka pansi pa gulu ili: 1) The microscopic agglutination test (MAT);2) ELISA;3) Mayeso a antibody osalunjika a fulorosenti (IFATs).Komabe, njira zonse zomwe tazitchulazi zimafuna malo apamwamba komanso akatswiri ophunzitsidwa bwino.
Leptospira IgG/IgM ndi mayeso osavuta a serological omwe amagwiritsa ntchito ma antigen kuchokera ku L. interrogans ndikuzindikira ma antibodies a IgG ndi IgM ku tizirombozi nthawi imodzi.Mayeso amatha kuchitidwa ndi osaphunzitsidwa kapena aluso pang'ono, opanda zida zovutirapo za labotale ndipo zotsatira zake zimapezeka mkati mwa mphindi 15.
MFUNDO
The Leptospira IgG/IgM Rapid Test Kit ndi lateral flow chromatographic
immunoassay.Kaseti yoyesera ili ndi: 1) burgundy colored conjugate pad yomwe ili ndi ma antigen a L. interrogans ophatikizidwa ndi golide wa colloid (Leptospira conjugates) ndi akalulu a IgG-golide conjugates, 2) nembanemba ya nitrocellulose yomwe ili ndi magulu awiri oyesera (M ndi G band) ndi gulu lowongolera (C band).M band imakutidwa kale ndi monoclonal anti-human IgM kuti izindikire anti-L.. interrogans IgM, G band imayikidwa kale ndi ma reagents kuti azindikire anti-L.interrogans IgG, ndipo C band idakutidwa kale ndi anti rabbit IgG.
Pamene chiwerengero chokwanira cha chitsanzo choyesera chaperekedwa mu chitsime cha kaseti, chitsanzocho chimasuntha ndi capillary action kudutsa kaseti.IgM anti-L.. interrogans ngati alipo mu chitsanzo adzamanga kwa Leptospira conjugates.The immunocomplex ndiye anagwidwa pa nembanemba ndi pre- TACHIMATA anti-anthu IgM antibody, kupanga burgundy mtundu M band, kusonyeza L.. interrogans IgM zotsatira zabwino mayeso.IgG anti-L.. interrogans ngati alipo mu chitsanzo adzamanga kwa Leptospira conjugates.The immunocomplex ndiye anagwidwa ndi pre- TACHIMATA reagents pa nembanemba, kupanga burgundy wachikuda G gulu, kusonyeza L.. interrogans IgG zotsatira zabwino mayeso.
Kusakhalapo kwa magulu aliwonse oyeserera (M ndi G) kukuwonetsa zotsatira zoyipa.Mayesowa ali ndi chowongolera chamkati (C band) chomwe chimayenera kuwonetsa gulu lamtundu wa burgundy la immunocomplex of goat anti rabbit IgG/rabbit IgG-gold conjugate mosasamala kanthu za kukula kwa mtundu pamagulu aliwonse oyesa.Apo ayi, zotsatira zake ndizolakwika ndipo chitsanzocho chiyenera kuyesedwanso ndi chipangizo china.
