Kufotokozera mwatsatanetsatane
Kuzindikira kwa antibody ya virus ya nkhosa kumapangidwa ndi microplate yomwe idakutidwa kale ndi antigen ya nyukiliya ya nkhosa, zolembera za ma enzyme ndi ma reagents ena othandizira, ndipo mfundo ya enzyme-linked immunoassay (ELISA) imagwiritsidwa ntchito kuzindikira ma antibody ankhope m'magazi. sampuli ya seramu ya nkhosa.Pakuyesa, seramu yowongolera ndi chitsanzo chomwe chiwunikiridwa chimawonjezedwa ku mbale ya microplate, ndipo ngati chitsanzocho chili ndi antibody yapox pox pambuyo pakukulitsidwa, imamangidwa ku antigen pa mbale ya microplate, ndi zigawo zina zomwe sizimangika. adzachotsedwa pambuyo kusamba;Kenako yonjezerani chikhomo cha enzyme kuti mumangirire ku antigen-antibody complex pa microplate plate;Zolemba za ma enzyme zosamangidwa zidachotsedwa ndikutsuka, ndipo gawo la gawo la TMB linawonjezeredwa ku zitsime, ndipo chopangidwa ndi buluu chinapangidwa ndi momwe ma conjugates a microplate, ndipo kuya kwa mtundu kumalumikizidwa bwino ndi kuchuluka kwa ma antibodies omwe ali mu chitsanzo.Pambuyo kuthetsa njira anawonjezera kuthetsa anachita, mankhwala anasanduka chikasu;Kuchuluka kwa kuyamwa pamachitidwe aliwonse kumatsimikiziridwa ndi owerenga ma microplate pamtunda wa 450 nm kuti adziwe ngati chitsanzocho chili ndi ma antibodies a pox pox.