Kuyesa kwa Antigen kwa Rota virus

Kutsekula m'mimba ndi chimodzi mwazinthu zomwe zimayambitsa kudwala kwa ana komanso kufa padziko lonse lapansi, zomwe zimapangitsa kuti anthu 2.5 miliyoni amafa chaka chilichonse.Matenda a Rotavirus ndi omwe amayambitsa matenda otsekula m'mimba kwambiri mwa makanda ndi ana osapitirira zaka zisanu, zomwe zimachititsa 40% -60% ya chifuwa chachikulu cha m'mimba ndikupangitsa kuti ana pafupifupi 500,000 amafa chaka chilichonse.Pofika zaka zisanu, pafupifupi mwana aliyense padziko lapansi ali ndi kachilombo ka rotavirus kamodzi kokha.Ndi matenda otsatirawa, kuyankha kwakukulu, heterotypic antibody kumayambitsa;choncho, akuluakulu sakhudzidwa kawirikawiri.Mpaka pano magulu asanu ndi awiri a rotavirus (magulu AG) adadzipatula ndipo amadziwika.Gulu A la rotavirus, lofala kwambiri, limayambitsa matenda opitilira 90% mwa anthu.Rotavirus imafalikira makamaka ndi njira yapakamwa, kuchokera kwa munthu kupita kwa munthu.Ma virus titers mu ndowe amafika pachimake matenda atangoyamba kumene, kenako amatsika.Nthawi yamakulitsidwe a matenda a rotavirus nthawi zambiri imakhala tsiku limodzi kapena atatu ndipo imatsatiridwa ndi gastroenteritis yomwe imakhala ndi masiku atatu kapena asanu ndi awiri.Zizindikiro za matendawa zimayamba kutsekula m'mimba pang'onopang'ono mpaka kutsekula m'mimba kwambiri ndi kutentha thupi ndi kusanza.Kuzindikira kwa matenda a rotavirus kumatha kupangidwa potsatira matenda a gastroenteritis chifukwa choyambitsa matenda otsekula m'mimba mwa ana.Posachedwapa, kuzindikira kwachindunji kwa matenda a rotavirus kwapezeka pozindikira kachilombo ka antigen mu chopondapo ndi njira za immunoassay monga latex agglutination assay, EIA, ndi lateral flow chromatographic immunoassay.Rotavirus Ag Rapid Test ndi lateral flow chromatographic immunoassay yomwe imagwiritsa ntchito ma antibodies apadera kuti azindikire moyenerera antigen ya rotavirus mu ndowe.Mayeso amatha kuchitidwa popanda zida zovuta za labotale, ndipo zotsatira zake zimapezeka mkati mwa mphindi 15.

Rotavirus Ag Rapid Test ndi lateral flow chromatographic immunoassay.

Mzere woyeserera uli ndi:

1) burgundy colored conjugate pad yokhala ndi anti-rotavirus antibody yolumikizidwa ndi golidi wa colloidal (anti-rotavirus conjugates) ndi anti-anti-antibody yolumikizidwa ndi golide wa colloidal.

2) mzere wa membrane wa nitrocellulose wokhala ndi mzere woyesera (T mzere) ndi mzere wowongolera (C mzere).

Mzere wa T umakhala wokutidwa ndi anti-rotavirus wina wa monoclonal, ndipo mzere wa C umakhala wokutidwa ndi anti-control line.