CHIDULE NDI KUTANTHAUZA MAYESERO
Chifuwa cha TB ndi matenda osatha, opatsirana omwe amayamba makamaka ndi M. TB hominis (Koch's bacillus), nthawi zina ndi M. TB bovis.Cholinga chachikulu cha mapapo, koma chiwalo chilichonse chingathe kutenga kachilomboka.
Chiwopsezo cha matenda a TB chatsika kwambiri m'zaka za zana la 20.Komabe, kutulukira kwaposachedwapa kwa mitundu yosaiŵala mankhwala1, makamaka kwa odwala AIDS2, kwatsitsimutsanso chidwi cha TB.Chiwerengero cha matenda chinanenedwa pafupifupi 8 miliyoni pachaka ndi imfa ya 3 miliyoni pachaka.Imfa idaposa 50% m'maiko ena aku Africa omwe ali ndi kachilombo ka HIV.
Kukayikitsa koyambirira kwachipatala ndi zomwe zapezedwa ndi ma radiographic, zomwe zimatsimikiziridwa ndi labotale pofufuza sputum ndi chikhalidwe ndi njira zachikhalidwe pakuzindikiritsa TB5,6 yogwira.Komabe, njirazi mwina zilibe mphamvu kapena zimatenga nthawi, makamaka sizoyenera kwa odwala omwe sangathe kutulutsa sputum, smear-negative, kapena omwe akuganiziridwa kuti ali ndi TB ya m'mapapo.
Mayeso a TB IgG/IgM Combo Rapid Test amapangidwa kuti achepetse zopinga izi.Mayesowa amazindikira IgM ndi IgG anti-M.TB mu seramu, plasma, kapena magazi athunthu mkati mwa mphindi 15.Zotsatira zabwino za IgM zimawonetsa matenda atsopano a M.TB, pomwe kuyankha kwabwino kwa IgG kukuwonetsa matenda am'mbuyomu kapena osatha.Pogwiritsa ntchito ma antigen enieni a M.TB, imazindikiranso IgM anti-M.TB mwa odwala omwe ali ndi katemera wa BCG.Komanso, mayeso angakhale
kuchitidwa ndi anthu osaphunzitsidwa kapena aluso ochepa popanda zida zovutirapo za labotale.
MFUNDO
TB IgG/IgM Rapid Test ndi lateral flow chromatographic immunoassay.Kaseti yoyesera imakhala ndi: 1) pad yamtundu wa burgundy yokhala ndi ma antigen a M.TB ophatikizidwa ndi golide wa colloid (M.TB conjugates) ndi ma conjugates a kalulu a IgG-golide, 2) mzere wa nitrocellulose wokhala ndi magulu awiri oyesera (M ndi G magulu ) ndi gulu lowongolera (C band).M band idakutidwa kale ndi monoclonal anti-human IgM kuti izindikire IgM anti- M.TB, G band idakutidwa kale ndi ma reagents kuti azindikire IgG anti-M.TB, ndipo C band ndi - yokutidwa ndi mbuzi anti-kalulu IgG.
Pamene chiwerengero chokwanira cha chitsanzo choyesera chaperekedwa mu chitsime cha kaseti, chitsanzocho chimasuntha ndi capillary action kudutsa kaseti.IgM anti-M.TB ngati ikupezeka pachitsanzoyo idzalumikizana ndi ma conjugates a M.TB.The immunocomplex kenako amagwidwa pa nembanemba ndi anti-munthu IgM antibody, kupanga burgundy mtundu M band, kusonyeza M.TB IgM zotsatira zabwino.IgG anti-M.TB, ngati ilipo pachitsanzocho, imalumikizana ndi ma conjugates a M.TB.The immunocomplex ndiye amagwidwa ndi reagents pre-kitamatidwa pa nembanemba, kupanga burgundy mtundu G band, kusonyeza M.TB IgG zotsatira zabwino.Kusakhalapo kwa magulu aliwonse oyeserera (M ndi G) kukuwonetsa zotsatira zoyipa.Mayesowa ali ndi chowongolera chamkati (C band) chomwe chimayenera kuwonetsa gulu lamtundu wa burgundy la immunocomplex la anti rabbit IgG/rabbit IgG-gold conjugate mosasamala kanthu za kukula kwa mtundu pamagulu aliwonse a T.Apo ayi, zotsatira zake ndizolakwika ndipo chitsanzocho chiyenera kuyesedwanso ndi chipangizo china.
